Improving Z-Tracking Accuracy in Two-Photon Single-Particle Tracking Microscope
نویسندگان
چکیده
منابع مشابه
Improving z-tracking accuracy in the two-photon single-particle tracking microscope.
Here, we present a method that can improve the z-tracking accuracy of the recently invented TSUNAMI (Tracking of Single particles Using Nonlinear And Multiplexed Illumination) microscope. This method utilizes a maximum likelihood estimator (MLE) to determine the particle's 3D position that maximizes the likelihood of the observed time-correlated photon count distribution. Our Monte Carlo simula...
متن کاملParticle Tracking in a 2-Photon Excitation Microscope
Introduction Within the past few years, single-particle tracking techniques have been increasingly used for studying biological systems. In contrast to large ensemble measurements, in which only average quantities are obtained, single-particle methods provide information about the distribution of molecular properties in inhomogeneous systems. Analysis of single molecule trajectories is useful t...
متن کاملThe single particle tracking system
In an optical tweezers system, the force measurement with a resolution less than pico-Newton can be achieved by precise measurement and analysis of the trapped particle trajectory. Typically, this single particle tracking technique is realized by a quadrant position sensor which detects the scattering lights of the trapping laser beam from the trapped particle. However, as the radius of the tra...
متن کامل3D orbital tracking in a modified two-photon microscope: an application to the tracking of intracellular vesicles.
The objective of this video protocol is to discuss how to perform and analyze a three-dimensional fluorescent orbital particle tracking experiment using a modified two-photon microscope(1). As opposed to conventional approaches (raster scan or wide field based on a stack of frames), the 3D orbital tracking allows to localize and follow with a high spatial (10 nm accuracy) and temporal resolutio...
متن کاملA New Modified Particle Filter With Application in Target Tracking
The particle filter (PF) is a novel technique that has sufficiently good estimation results for the nonlinear/non-Gaussian systems. However, PF is inconsistent that caused mainly by loss of particle diversity in resampling step and unknown a priori knowledge of the noise statistics. This paper introduces a new modified particle filter called adaptive unscented particle filter (AUPF) to overcome th...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2016
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2015.11.3392